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In vitro multiplication of Panagrolaimus sp. (Nematoda: Rhabditida)

Mehrnoush Aminisarteshnizi

Abstract


Aim: Artificial culture systems of free-living nematodes can provide much information. Under the controlled condition, life cycles of free-living nematodes influence by temperature and food levels. The aim of the study was to find the best substrate for multiplication of the Panagrolaimus in the laboratory condition.

Materials and methods: This study evaluated nine different media for in vitro mass production of Panagrolaimus. The media was including WA 1.5%, WA 1.5% + Goat butter oil, WA 1.5% + K2HPO4, SDA (Sabouraud 4% Dextrose Agar), SDA+ K2HPO4, SDA+ Goat butter oil, NA, NA+ K2HPO4, and NA+ Goat butter oil. The nematodes were counted after four, seven, and fourteen-day. The experiment was repeated two times.

Results: Maximum multiplication of all the tested species was observed in WA 1.5% + Goat butter oil as 612.3 after 7 days. These results indicated that goat butter oil was a better medium for the laboratory multiplication of Panagrolaimus.

Conclusion: The result indicated that goat butter is a good option to add the media to multiply the free-living nematodes in laboratory conditions.

Keywords


free-living nematode, Panagrolaimus, goat butter oil, laboratory, multiplication

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